{"id":2378,"date":"2020-03-27T21:16:14","date_gmt":"2020-03-27T21:16:14","guid":{"rendered":"http:\/\/sites.uw.edu\/lallyj2\/?page_id=77"},"modified":"2021-06-07T01:10:03","modified_gmt":"2021-06-07T08:10:03","slug":"equipment","status":"publish","type":"page","link":"https:\/\/sites.bioe.uw.edu\/giachelli\/equipment\/","title":{"rendered":"Equipment and Resources"},"content":{"rendered":"<h4><a href=\"https:\/\/sites.uw.edu\/lallyj2\/files\/2020\/04\/IMG_0404.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-224 size-medium\" src=\"https:\/\/sites.uw.edu\/lallyj2\/files\/2020\/04\/IMG_0404-225x300.jpg\" alt=\"\" width=\"225\" height=\"300\" srcset=\"https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0404-225x300.jpg 225w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0404-768x1024.jpg 768w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0404-1152x1536.jpg 1152w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0404-375x500.jpg 375w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0404-750x1000.jpg 750w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0404-1140x1520.jpg 1140w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0404.jpg 1440w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a>iBright FL1000<\/h4>\n<p>The iBright FL1000 is a digital Western blot and gel imager complete with data analysis software. Using a green LEDbased transilluminator as well as two epi-LEDs for fluorescence imaging applications, the iBright can be used for imaging and documenting chemiluminescent western blots and colorimetrically stained protein and nucleic acid gels, fluorescent gel and blot imaging capability using visible and near-IR excitation and emission channels. This model has five fluorescence channels, three in the visible fluorescence range and two in the near-IR fluorescence range. Up to four channels can be multiplexed when imaging a single western blot or set of four mini blots.<\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/sites.uw.edu\/lallyj2\/files\/2020\/04\/IMG_0405.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-227 size-medium\" src=\"https:\/\/sites.uw.edu\/lallyj2\/files\/2020\/04\/IMG_0405-225x300.jpg\" alt=\"\" width=\"225\" height=\"300\" srcset=\"https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0405-225x300.jpg 225w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0405-768x1024.jpg 768w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0405-1152x1536.jpg 1152w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0405-375x500.jpg 375w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0405-750x1000.jpg 750w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0405-1140x1520.jpg 1140w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0405.jpg 1440w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><\/p>\n<h4>Dedicated Tissue Culture Suite<\/h4>\n<p>At left is\u00a0our in-lab, dedicated tissue culture suite connected to the main lab space. Inside are two class II, type A2 biological safety cabinets, 4 incubators, a dedicated water bath, several freezers, a water bath, and centrifuge. This self contained space helps to reduce cross contamination from the rest of the lab for optimal results. Inside, the room is well lit and well ventilated.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/sites.uw.edu\/lallyj2\/files\/2020\/04\/IMG_0410.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-225 size-medium\" src=\"https:\/\/sites.uw.edu\/lallyj2\/files\/2020\/04\/IMG_0410-225x300.jpg\" alt=\"\" width=\"225\" height=\"300\" srcset=\"https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0410-225x300.jpg 225w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0410-768x1024.jpg 768w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0410-1152x1536.jpg 1152w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0410-375x500.jpg 375w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0410-750x1000.jpg 750w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0410-1140x1520.jpg 1140w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0410.jpg 1440w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><\/p>\n<h4>Leica CM1850 Cryostat<\/h4>\n<p>This cryostat features a well lit and spacious chamber work area capable of cutting sections as small as 3um. It has several helpful ease of use features including lateral blade displacement for use of the whole blade without repositioning the sample, a motorized coarse feed, and rapid specimen freezing as low as -60C.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/sites.uw.edu\/lallyj2\/files\/2020\/04\/IMG_0407.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-226 size-medium\" src=\"https:\/\/sites.uw.edu\/lallyj2\/files\/2020\/04\/IMG_0407-225x300.jpg\" alt=\"\" width=\"225\" height=\"300\" srcset=\"https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0407-225x300.jpg 225w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0407-768x1024.jpg 768w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0407-1152x1536.jpg 1152w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0407-375x500.jpg 375w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0407-750x1000.jpg 750w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0407-1140x1520.jpg 1140w, https:\/\/sites.bioe.uw.edu\/giachelli\/wp-content\/uploads\/sites\/34\/2020\/04\/IMG_0407.jpg 1440w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><\/p>\n<h4>Applied Biosystems qPCR Cycler<\/h4>\n<p>Reliable machine that handles our qPCR needs.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>iBright FL1000 The iBright FL1000 is a digital Western blot and gel imager complete with data analysis software. Using a green LEDbased transilluminator as well as two epi-LEDs for fluorescence imaging applications, the iBright can be used for imaging and documenting chemiluminescent western blots and colorimetrically stained protein and nucleic acid gels, fluorescent gel and blot imaging capability using visible and near-IR excitation and emission channels. This model has five fluorescence channels, three in the visible fluorescence range and two&#8230;<\/p>\n","protected":false},"author":1,"featured_media":2529,"parent":0,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"templates\/template-small-hero.php","meta":{"footnotes":""},"class_list":["post-2378","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/sites.bioe.uw.edu\/giachelli\/wp-json\/wp\/v2\/pages\/2378","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.bioe.uw.edu\/giachelli\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.bioe.uw.edu\/giachelli\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.bioe.uw.edu\/giachelli\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.bioe.uw.edu\/giachelli\/wp-json\/wp\/v2\/comments?post=2378"}],"version-history":[{"count":0,"href":"https:\/\/sites.bioe.uw.edu\/giachelli\/wp-json\/wp\/v2\/pages\/2378\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sites.bioe.uw.edu\/giachelli\/wp-json\/wp\/v2\/media\/2529"}],"wp:attachment":[{"href":"https:\/\/sites.bioe.uw.edu\/giachelli\/wp-json\/wp\/v2\/media?parent=2378"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}